| Record no |
2011032225685 |
| Activity status |
Dead or Administration by others |
| Working Title |
AN IMPROVED PROCESS FOR PRODUCTION OF 6-AMINO PENICILLANIC ACID (6-APA) USING IMMOBILIZED PENICILLIN G ACYLASE MULTI STAGE STIRRED TANK SYSTEM |
| Working Inventors |
KULKARNI BS;SUDAKARAN VK;DESHPANDE BS;SHEWALE JG;NAIK SR; |
| Contributing institutions |
NCL |
| Administered by |
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| Ownership |
|
| Categories > Subjects |
Chemical engineering and process development |
| Categories > Portfolio |
Industrial biotechnology/enzymology |
| Disclosure number |
|
| NCL No |
1992-NCL-0025 |
| CSIR No |
1992-NF-0198
|
| Priority date |
12-10-1992 |
| Country |
IN |
| Application number |
1174DEL1992 |
| Provisional filing date |
12-10-1992 |
| Complete filing date |
02-02-1994 |
| Publication status |
Yes |
| Publication date |
|
| Publication no |
|
| Granted number |
181300 |
| Granted date |
05-14-1999 |
| Final title |
AN IMPROVED PROCESS FOR PRODUCTION OF 6-AMINO PENICILLANIC ACID (6-APA) USING IMMOBILIZED PENICILLIN G ACYLASE MULTI STAGE STIRRED TANK SYSTEM |
| Final Inventors |
KULKARNI BS;SUDAKARAN VK;DESHPANDE BS;SHEWALE JG;NAIK SR; |
| Abstract |
An improved proceaa for the production of 0 amino penleillanio
« APA uaing lranobilixe« penicillin O acylase in multl etaoe
•tirred tenlc eyetem which eonffiriaee o« auapendlng iMMoblllced
penicillin O acylaao »uch aa here in described in 0.03 M phoeph
te buffer at pH in the range 1.« S«0 in a Reactor. adding t »
the aaid euapenslon aolution of beniyl penicillin and ealta
thereof agitating the resultant mixture at 60 80 rpm at 37UC
Maintaining the pH of the reaction mixture between 7.6 8.0 by
neutralisation of liberated phenylacetlc acid with 1.0 N anmoni«
solution transferring the hydrolyaate after 30 ninutea to a
second Reactor adding .InRobillsed penicillin a acylase to the
second Reactor agitating the resultant mixture at 0«80 rpm at
37« ct maintaining the pH of the reaction mixture between 7 « 8.0
by neutralization of liberated phonyVacetic acid with 2.0 H
anmonia solution discharging the hydrolysate from the second
Reactor and isolating «• » e WA from the hydroiyeate each an
herein described 1 |
| Case status |
Granted + Expired |
| Licensing status |
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| Licensing case no |
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| Client name |
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| Lead inventors |
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| Categories > Industry > Primary |
|
| Categories > Industry > other |
|